A batch-type concentration and purification procedure for poliovirus complement fixing antigen.
نویسندگان
چکیده
* Culture 7 days old. Flasks of sulfur medium were inoculated with T. thiooxidans and incubated at 28 C as shaken cultures. At several incubation periods, determinations were made of the surface tension of the culture medium freed of elemental sulfur, before and after removal of cells. The surface tension of the sterile culture medium (72.0 i 0.5 dynes/ cm) was practically identical to that of distilled water, whereas the surface tension of the culture medium dropped to approximately 49 dynes/cm during the first few days of bacterial growth and remained at this or at a lower level (Fig. 1). The surface tension of medium containing active cells dropped more rapidly than that of medium with cells removed. Wetting and sedimentation of sulfur, and acidity (titer), which are proportional to growth, increased with decrease in surface tension. Ultraviolet absorption of a 10-day-old culture solution freed of cells, measured by a Beckman DU spectrophotometer, showed a round peak at 255 to 260 m,.. The solution gave a positive ninhydrin test. An acid hydrolyzate gave a stronger test, as well as a strong test for cysteine and a positive Folin-Ciocalteau reaction. Thus, during growth of the bacterium, organic surfaceactive substances, including amino acids and polypeptides, appear in the medium. These are probably responsible for wetting of the sulfur. Since the substances appear during the period of rapid growth, they apparently arise at least in part through leakage from actively metabolizing cells.
منابع مشابه
Heterogeneity of apparently complete poliovirus particles.
Tumilowicz, Joseph J. (Children's Hospital of Philadelphia, Philadelphia, Pa.), and Klaus Hummeler. Heterogeneity of apparently complete poliovirus particles. J. Bacteriol. 87:1105-1113. 1964.-A chromatographic procedure was developed for separating the N from the H complement-fixing antigen of poliovirus. This procedure concomitantly effected some separation of classes of N particle. The ratio...
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عنوان ژورنال:
- Journal of bacteriology
دوره 82 شماره
صفحات -
تاریخ انتشار 1961